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  • 食品微生物实验室设计应该按照什么来做?
    来源: 浏览: 发布日期:2020-04-28
    清洁区
    Clean Zone
    清洁区有分为办公室、休息室、培育基配制室与试剂储藏室。这个区域内不行带细菌查验标本进入。
    The cleaning area is divided into office, rest room, culture medium preparation room and reagent storage room. This area is not allowed to enter with bacteria inspection samples.
    操作区
    Operation area
    1、微生物试验室操作区要注意洁净度,防止交叉污染,操作区要单独分开。在进入试验室内时要换鞋,送标本人员不得进入操作区,操作区地面要用专用的清洁东西整理,至少,每天拖1次地,消毒1次,在进入试验室或出操作区的时分要先紫外线照耀30-60min。
    1. The operation area of the microbiological laboratory shall be clean to prevent cross contamination, and the operation area shall be separated separately. Shoes shall be changed when entering the laboratory. The specimen delivering personnel shall not enter the operation area. The ground of the operation area shall be cleaned with special cleaning things. At least, the ground shall be dragged and disinfected once a day. When entering the laboratory or leaving the operation area, ultraviolet light shall be used for 30-60min first.
    2、注意光线的问题,在培育细小及血清试验凝聚颗粒时,都需求满意的光线,操作区除了惯例的照明外,还能够装置一个操作台灯,方便观察试验室的准确度。
    2. Pay attention to the problem of light. When cultivating small and serum test agglomerated particles, satisfactory light is required. Besides conventional lighting, an operation lamp can also be installed in the operation area to facilitate the observation of the accuracy of the laboratory.
    3、试验要在生物安全柜中操作,既确保了环境不受污染,也保证了工作人员的安全。
    3. The test should be operated in the biosafety cabinet, which not only ensures the environment not to be polluted, but also ensures the safety of the staff.
    4、因为无菌操作的要求,试验过程中常常使用酒精灯,因此,微生物学试验室不能装置吊扇。为了到达试验所需的适合温度,尤其是满意某些仪器对温度湿度的要求,试验室应装置空调。
    4. Because of the requirements of aseptic operation, alcohol lamp is often used in the test process, so the microbiology laboratory can not install ceiling fan. In order to reach the appropriate temperature for the test, especially to satisfy the requirements of some instruments for temperature and humidity, the test room should be equipped with air conditioning.
    5、依据仪器设备要求,必要时装备不间断电源。
    5. According to the requirements of instruments and equipment, uninterruptible power supply shall be equipped if necessary.
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    6、操作区内须设置水源。用于标本处理(如细菌染色) 的水槽与工作人员洗手用的水槽不能混用。
    6. Water source shall be set in the operation area. The sink used for specimen processing (such as bacterial staining) shall not be mixed with the sink used for staff's hand washing.
    7、污染物处理:操作区须备有消毒缸,以处理沾有活菌的玻片等污染物品。查验剩余的标本及使用过的带菌平板、试管均须集中地点安全放置,经消毒灭菌处理后再洗刷或丢弃。
    7. Pollutant treatment: the operation area must be equipped with a disinfection tank to deal with the contaminated items such as slides stained with live bacteria. The remaining samples, used plates and test tubes shall be placed in a centralized place safely, washed or discarded after disinfection and sterilization.
    无菌区
    Aseptic area
    1、无菌室应完全关闭,进出无菌室至少要经两道门,中间隔有缓冲间,无菌室与外间设置一个可开闭的窗口,用于传递用具。
    1. The sterile room shall be completely closed. The access to the sterile room shall pass through at least two doors, with a buffer room in the middle. A window which can be opened and closed shall be set between the sterile room and the outer room for the delivery of utensils.
    2、无菌室必须保持整洁。工作人员进入无菌室应换专用鞋、专用衣。无菌室使用前须用紫外线消毒30 min ,操作完毕后清洁台面,再用紫外线消毒30 min 。定时用乳酸或甲醛熏蒸,彻底消毒。
    2. The sterile room must be kept clean and tidy. When entering the sterile room, the staff should change the special shoes and clothes. The sterile room shall be sterilized with ultraviolet for 30 minutes before use, and the table top shall be cleaned after operation, and then sterilized with ultraviolet for 30 minutes. Regularly fumigate with lactic acid or formaldehyde and thoroughly disinfect.
    3、无菌室仅用于培育基分装等无菌操作,不能进行有菌标本的操作。操作人员操作时应严格关门,并戴好专用的口罩、帽子。
    3. The aseptic room is only used for aseptic operations such as sub packaging of culture medium, and cannot be used for operations with bacteria samples. Operators shall close the door strictly and wear special masks and hats.
    4、无菌室内应有空气过滤装置,并装置空调。
    4. The sterile room shall be equipped with air filtration device and air conditioner.
     
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