无菌手术室的清洁度应达到10,000，室内温度应保持在20°C-24°C，湿度应保持在45％?60％，洁净工作台的清洁度应达到100。 每月应在无菌室内检查菌落数量。 在无菌室或无尘工作台打开的状态下，取出许多内径为90mm的无菌陪替氏培养皿，并且将无菌的融化并冷却至约45℃的平板计数琼脂无菌注入。 放置并固化15 mL培养基，然后在36°C±1°C的培养箱中放置48小时。 消毒后，放置3到5个板，放置在工作位置的左右，左右位置，并露出盖板。 一分钟后，将其置于36°C±1°C的培养箱中48小时，然后取出进行检查。 100级洁净区中的平板细菌平均数量不得超过1个菌落，而10000级洁净室中的平均洁净室则不得超过3个菌落。 如果超过限值，则应对无菌室进行彻底消毒，直到再次检查合格为止。
The cleanliness of aseptic operating room should reach 10,000, the indoor temperature should be kept at 20 C - 24 C, the humidity should be kept at 45% 60%, and the cleanliness of clean worktable should reach 100. The number of colonies should be checked monthly in the sterile room. When the sterile chamber or dust-free workbench is open, many 90 mm sterile Petri dishes are taken out, and the sterile plates are melted and cooled to about 45 C. The agar is counted and injected sterile. Place and solidify 15 mL culture medium, and then place it in a incubator of 36 C + 1 C for 48 hours. After disinfection, place 3 to 5 boards around the working position, left and right, and expose the cover. After one minute, they were placed in a incubator of 36 C + 1 C for 48 hours, and then removed for examination. The average number of bacteria on the plate in the 100 grade clean area should not exceed one colony, while the average number of bacteria in the 10 000 grade clean room should not exceed three colony. If the limit value is exceeded, the sterile room shall be thoroughly sterilized until it is qualified for re inspection.
无菌室应保持整洁。 仅将必需的检查工具（例如酒精灯，酒精棉，打火机，镊子，接种环，剪刀和玻璃笔）存放在室内。 严禁堆放杂物以防止污染。 无菌室应提供工作浓度的消毒剂，例如5％的甲酚溶液，75％的酒精，0.1％的新螯合剂溶液等。 需要带入无菌室的仪器，药品，玻璃器皿等应紧密包装或通过适当方法进行灭菌。 例如，将培养皿和吸管在干燥箱中在160℃至170℃下灭菌1h至2h，并且将生理盐水通过高压釜在121℃下灭菌20分钟。 在使用无菌室之前，必须将无菌室和缓冲室的紫外线灯打开30分钟以上，并同时打开净化风扇进行通风。 紫外线灯关闭后仅半小时即可进入无菌室工作。 检查样品后，应及时清洁剩余的样品以及各种药物和玻璃器皿，并应打开所有紫外线灯并对其消毒30分钟。
The sterile room should be kept clean and tidy. Store only the necessary inspection tools (e.g. alcohol lamps, alcohol cotton, lighters, tweezers, inoculation rings, scissors and glass pens) indoors. It is strictly forbidden to pile up sundries to prevent pollution. The sterile room should provide disinfectants of working concentration, such as 5% cresol solution, 75% alcohol, 0.1% new chelating agent solution, etc. Instruments, medicines and glassware that need to be brought into the sterile room should be packaged tightly or sterilized by appropriate methods. For example, Petri dishes and pipettes were sterilized for 1 to 2 hours in a drying chamber at 160 to 170 degrees Celsius, and saline was sterilized for 20 minutes in an autoclave at 121 degrees Celsius. Before using the sterile chamber, the ultraviolet lamp in the sterile chamber and buffer chamber must be turned on for more than 30 minutes, and the purifying fan must be turned on for ventilation at the same time. Ultraviolet light can enter sterile room only half an hour after it is turned off. After checking the samples, the remaining samples and various medicines and glassware should be cleaned in time, and all ultraviolet lamps should be turned on and disinfected for 30 minutes.
无菌室通常是在微生物实验室专门设置的小室。 面积不要太大，大约4m2?5m2，高度大约2.5m。 无菌室外室应设有缓冲室，净化空气淋浴室和更衣室。 无菌室和缓冲室都必须密封。 室内设备必须装有空气过滤器。 紫外线灯安装在无菌室和缓冲室中，无菌室中的紫外线是从工作表面发出的。 无菌室的地板和墙壁必须平坦，不易隐藏污垢，且易于清洁。 工作台的桌面必须水平。 进入无菌室的工人应在更衣室里穿着无菌的衣服，帽子和鞋子，经过洁净的空气淋浴，进入缓冲室，然后进入无菌室进行检查。
The sterile room is usually a small room specially set up in the microbiological laboratory. The area is not too large, about 4 M2 5 m2, and the height is about 2.5 m2. Sterile outdoors should be equipped with buffer rooms, clean air showers and dressing rooms. The sterile room and buffer room must be sealed. Indoor equipment must be equipped with air filters. Ultraviolet lamp is installed in sterile chamber and buffer chamber. Ultraviolet radiation in sterile chamber is emitted from working surface. The floor and wall of the sterile room must be flat, dirt can not be hidden easily, and it is easy to clean. The desktop of the workbench must be level. Workers entering the sterile room should wear sterile clothes, hats and shoes in the dressing room, take a clean air shower, enter the buffer room, and then enter the sterile room for inspection.